采用硅光电倍增管阵列扩展探测实现非线性荧光显微技术中散射光子探测信号的增强

施汝恒; 靳程; 刘驰; 孔令杰

doi:10.1631/FITEE.2000410

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采用硅光电倍增管阵列扩展探测实现非线性荧光显微技术中散射光子探测信号的增强

常规文章 | Updated：2022-06-06

- 采用硅光电倍增管阵列扩展探测实现非线性荧光显微技术中散射光子探测信号的增强
- Enhanced collection of scattered photons in nonlinear fluorescence microscopy by extended epi-detection with a silicon photomultiplier array
- 信息与电子工程前沿（英文） 2021年22卷第10期页码：1289-1298
- Affiliations：
  
  State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, China
  IDG/McGovern Institute for Brain Research, Tsinghua University, Beijing 100084, China
- Author bio：
  
  [ "", "Ruheng SHI, first author of this invited paper, received his BS degree in optoelectronic information science and engineering from Sichuan University, China, in 2018. He is now a PhD candidate at the Department of Precision Instrument, Tsinghua University, Beijing, China. His research focuses on optical imaging" ]
  [ "", "Lingjie KONG, corresponding author of this invited paper, received his BS degree from the Department of Optical Communication, Jilin University, China, in 2007, and his PhD degree from the Department of Precision Instrument, Tsinghua University, China, in 2012. He is now an associate professor at the Department of Precision Instrument, Tsinghua University, and a PI at the IDG/McGovern Institute for Brain Research, Tsinghua University. His research focuses on the interdisciplinary study of optoelectronics and biomedicine, aiming to motivate biomedical study by developing novel optical techniques" ]
- Funds：
  
  the National Natural Science Foundation of China(61831014);the National Natural Science Foundation of China(61771287);the Tsinghua University Initiative Scientific Research Program, China(20193080076);the Graduate Education Innovation Grants, Tsinghua University, China(201905J003)
- DOI：10.1631/FITEE.2000410
  中图分类号： O439
- 收稿：2020-08-12，
  
  修回：2020-12-24，
  
  网络出版：2021-01-29，
  
  纸质出版：2021-10
- Accepted：
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施汝恒, 靳程, 刘驰, 等. 采用硅光电倍增管阵列扩展探测实现非线性荧光显微技术中散射光子探测信号的增强[J]. 信息与电子工程前沿（英文）, 2021,22(10):1289-1298.

SHI Ruheng, JIN Cheng, LIU Chi, et al. Enhanced collection of scattered photons in nonlinear fluorescence microscopy by extended epi-detection with a silicon photomultiplier array[J]. Frontiers of Information Technology & Electronic Engineering, 2021, 22(10): 1289-1298.
施汝恒, 靳程, 刘驰, 等. 采用硅光电倍增管阵列扩展探测实现非线性荧光显微技术中散射光子探测信号的增强[J]. 信息与电子工程前沿（英文）, 2021,22(10):1289-1298. DOI： 10.1631/FITEE.2000410.

SHI Ruheng, JIN Cheng, LIU Chi, et al. Enhanced collection of scattered photons in nonlinear fluorescence microscopy by extended epi-detection with a silicon photomultiplier array[J]. Frontiers of Information Technology & Electronic Engineering, 2021, 22(10): 1289-1298. DOI： 10.1631/FITEE.2000410.

摘要

为提高非线性荧光显微技术在活体成像中的信号探测能力，人们常采用基于非退扫描的背向探测策略。然而，由于生物样本中存在严重散射，物镜前孔径处大部分荧光光子辐射角度超出物镜收集角范围，因而无法被探测。本文提出一种将硅光电倍增管阵列放置在物镜前孔径处以增强非线性显微镜对散射光子探测能力的扩展探测方案。通过数值仿真说明了扩展探测在对人体皮肤和小鼠大脑（透过颅窗及完整颅骨）进行多光子荧光成像的信号增强情况。例如，在人类皮肤60 µm成像深度处使用红色染料标记情况下，使用10×，0.6NA物镜进行成像，扩展探测方案引入的信号增强可达约150%。本文论证了扩展探测是一种灵活、广泛适用于非线性荧光显微镜增强探测信号的技术。

Abstract

To maximize signal collection in nonlinear optical microscopy

non-descanned epi-detection is generally adopted for in vivo imaging. However

because of severe scattering in biological samples

most of the emitted fluorescence photons go beyond the collection angles of objectives and thus cannot be detected. Here

we propose an extended detection scheme to enhance the collection of scattered photons in nonlinear fluorescence microscopy using a silicon photomultiplier array ahead of the front apertures of objectives. We perform numerical simulations to demonstrate the enhanced fluorescence collection via extended epi-detection in the multi-photon fluorescence imaging of human skin and mouse brain through craniotomy windows and intact skulls. For example

with red fluorescence emission at a depth of 600 μm in human skin

the increased collection can be as much as about 150% with a 10×

0.6-NA objective. We show that extended epi-detection is a generally applicable

feasible technique for use in nonlinear fluorescence microscopy to enhance signal detection.

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