后胚胎期小鼠外膝体和上丘双眼分离的发育

Yu-qing CHEN; Yu-pu DIAO; Jing-gang DUAN; Li-yuan CUI; Jia-yi ZHANG

doi:10.1631/jzus.C1400153

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后胚胎期小鼠外膝体和上丘双眼分离的发育

脑机接口与半机器人智能专题 | Updated：2022-05-19

- 后胚胎期小鼠外膝体和上丘双眼分离的发育
- Time-dependent changes in eye-specific segregation in the dorsal lateral geniculate nucleus and superior colliculus of postnatal mice
- 信息与电子工程前沿（英文） 2014年15卷第10期页码：807-812
- Affiliations：
  
  Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China
- Author bio：
  
  Jia-yi ZHANG, E-mail: jiayizhang@fudan.edu.cn
- Funds：
  
  Project supported by the Shanghai Postdoctoral Scientific Program, the National Natural Science Foundation of China (No. 31271158), the Doctoral Program of Higher Education from Ministry of Education of China, the Innovation Program of Shanghai Municipal Education Commission (No. 13ZZ002), the Shanghai Municipal Commission of Health and Family Planning, Science and Technology Commission of Shanghai Municipality (Nos. 12ZR1441000 and 13PJ1401000), and the Young 1000 Plan
- DOI：10.1631/jzus.C1400153
  中图分类号： TP183;Q42
- 收稿：2014-04-26，
  
  修回：2014-;9-17，
  
  纸质出版：2014-10
- Accepted：
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后胚胎期小鼠外膝体和上丘双眼分离的发育[J]. 信息与电子工程前沿（英文）, 2014,15(10):807-812.

Yu-qing CHEN, Yu-pu DIAO, Jing-gang DUAN, et al. Time-dependent changes in eye-specific segregation in the dorsal lateral geniculate nucleus and superior colliculus of postnatal mice[J]. Frontiers of Information Technology & Electronic Engineering, 2014, 15(10): 807-812.
后胚胎期小鼠外膝体和上丘双眼分离的发育[J]. 信息与电子工程前沿（英文）, 2014,15(10):807-812. DOI： 10.1631/jzus.C1400153.

Yu-qing CHEN, Yu-pu DIAO, Jing-gang DUAN, et al. Time-dependent changes in eye-specific segregation in the dorsal lateral geniculate nucleus and superior colliculus of postnatal mice[J]. Frontiers of Information Technology & Electronic Engineering, 2014, 15(10): 807-812. DOI： 10.1631/jzus.C1400153.

摘要

研究目的

在小鼠视觉系统中，外膝体和上丘是自视网膜信息传递的两个重要中转站，而小鼠外膝体和上丘的结构特点之一是具有双眼分离的现象。本文意在系统地研究后胚胎期小鼠外膝体和上丘双眼分离的发育情况。

创新要点

首先，对后胚胎期上丘双眼分离随时间的发育进行了系统研究；其次，在同一个系统中，采用相同的实验方法和分析方法，同时研究外膝体和上丘中双眼分离的发育，使横向比较成为可能。

方法提亮

将偶联荧光染料的CTB进行眼内注射，48小时后灌流切鼠脑，利用Matlab程序分析同侧、异侧投射所占比例以及两者投射相重叠的比例。

重要结论

（1）在对外膝体和上丘双眼分离发育的整个过程中，同侧投射和同侧异侧重叠投射的比例都显著降低，与之相比，异侧投射所占比例的变化并非单向，且变化幅度小很多。（2）整个发育过程中，可将外膝体和上丘中双眼分离的发育分为两个阶段：快速发育阶段和趋缓的精细发育阶段。这两个阶段在外膝体和上丘中所需时间不同；实验数据表明，丘双眼分离的发育早于外膝体。

Abstract

Eye-specific segregation in the dorsal lateral geniculate nucleus (dLGN) and superior colliculus (SC) starts from the embryonic stage and continues to develop postnatally until eye-opening in mice. However

there have been few systematic studies on the details of this developmental process. Here

we carried out time-dependent studies of eye-specific segregation in the dLGN and SC. Our results demonstrated that the development of eye-specific segregation in the SC is completed before postnatal day 12 (P12)

which is earlier than in the dLGN (P20). During the whole period

ipsilateral and overlapping axonal projections decreased continuously in both the dLGN and SC. On the other hand

contralateral axonal projections showed little change

except for a slight decrease between P8 and P20 in the dLGN.

关键词

Keywords

references

A. Bansal , , , J.H. Singer , , , B.J. Hwang , , , 等 . . Mice lacking specific nicotinic acetylcholine receptor subunits exhibit dramatically altered spontaneous activity patterns and reveal a limited role for retinal waves in forming ON and OFF circuits in the inner retina . . J. Neurosci. , , 2000 . . 20 ( ( 20 ): ): 7672 - - 7681 . . . .

A.G. Blankenship , , , M.B. Feller . . Mechanisms under- lying spontaneous patterned activity in developing neural circuits . . Nat. Rev. Neurosci. , , 2010 . . 11 ( ( 1 ): ): 18 - - 29 . . DOI: 10.1038/nrn2759 http://doi.org/10.1038/nrn2759 . .

F. Bouzioukh , , , G. Daoudal , , , J. Falk , , , 等 . . Semaphorin3A regulates synaptic function of differentiated hippocampal neurons . . Eur. J. Neurosci. , , 2006 . . 23 ( ( 9 ): ): 2247 - - 2254 . . DOI: 10.1111/j.1460-9568.2006.04783.x http://doi.org/10.1111/j.1460-9568.2006.04783.x . .

J.M. Brooks , , , J. Su , , , C. Levy , , , 等 . . A molecular mechanism regulating the timing of corticogeniculate innervation . . Cell Reports , , 2013 . . 5 ( ( 3 ): ): 573 - - 581 . . DOI: 10.1016/j.celrep.2013.09.041 http://doi.org/10.1016/j.celrep.2013.09.041 . .

D.A. Butts , , , P.O. Kanold , , , C.J. Shatz . . . A burst-based "Hebbian" learning rule at retinogeniculate synapses links retinal waves to activity-dependent refinement . . PLoS Biol. , , 2007 . . 5 ( ( 3 ): ): e61 DOI: 10.1371/journal.pbio.0050061 http://doi.org/10.1371/journal.pbio.0050061 . .

K. Deisseroth . . Optogenetics . . Nat. Methods , , 2011 . . 8 ( ( 1 ): ): 26 - - 29 . . DOI: 10.1038/nmeth.f.324 http://doi.org/10.1038/nmeth.f.324 . .

J. Demas , , , S.J. Eglen , , , R.O. Wong . . Developmental loss of synchronous spontaneous activity in the mouse retina is independent of visual experience . . J. Neurosci. , , 2003 . . 23 ( ( 7 ): ): 2851 - - 2860 . . . .

M. Furman , , , H.P. Xu , , , M.C. Crair . . Competition driven by retinal waves promotes morphological and functional synaptic development of neurons in the superior colliculus . . J. Neurophysiol. , , 2013 . . 110 ( ( 6 ): ): 1441 - - 1454 . . DOI: 10.1152/jn.01066.2012 http://doi.org/10.1152/jn.01066.2012 . .

M.G. Hanson , , , L.T. Landmesser . . Normal patterns of spontaneous activity are required for correct motor axon guidance and the expression of specific guidance molecules . . Neuron , , 2004 . . 43 ( ( 5 ): ): 687 - - 701 . . DOI: 10.1016/j.neuron.2004.08.018 http://doi.org/10.1016/j.neuron.2004.08.018 . .

A.D. Huberman , , , C.M. Niell . . What can mice tell us about how vision works . . Trends Neurosci. , , 2011 . . 34 ( ( 9 ): ): 464 - - 473 . . DOI: 10.1016/j.tins.2011.07.002 http://doi.org/10.1016/j.tins.2011.07.002 . .

A.D. Huberman , , , M.B. Feller , , , B. Chapman . . Mechanisms underlying development of visual maps and receptive fields . . Ann. Rev. Neurosci. , , 2008 . . 31 ( ( 1 ): ): 479 - - 509 . . DOI: 10.1146/annurev.neuro.31.060407.125533 http://doi.org/10.1146/annurev.neuro.31.060407.125533 . .

L. Jaubert-Miazza , , , E. Green , , , F.S. Lo , , , 等 . . Structural and functional composition of the developing retinogeni- culate pathway in the mouse . . Vis. Neurosci. , , 2005 . . 22 ( ( 5 ): ): 661 - - 676 . . DOI: 10.1017/S0952523805225154 http://doi.org/10.1017/S0952523805225154 . .

F.M. Rossi , , , T. Pizzorusso , , , V. Porciatti , , , 等 . . Requirement of the nicotinic acetylcholine receptor beta 2 subunit for the anatomical and functional development of the visual system . . PNAS , , 2001 . . 98 ( ( 11 ): ): 6453 - - 6458 . . DOI: 10.1073/pnas.101120998 http://doi.org/10.1073/pnas.101120998 . .

W. Wei , , , A.M. Hamby , , , K. Zhou , , , 等 . . Development of asymmetric inhibition underlying direction selectivity in the retina . . Nature , , 2011 . . 469 ( ( 7330 ): ): 402 - - 406 . . DOI: 10.1038/nature09600 http://doi.org/10.1038/nature09600 . .

H.P. Xu , , , M. Furman , , , Y.S. Mineur , , , 等 . . An instructive role for patterned spontaneous retinal activity in mouse visual map development . . Neuron , , 2011 . . 70 ( ( 6 ): ): 1115 - - 1127 . . DOI: 10.1016/j.neuron.2011.04.028 http://doi.org/10.1016/j.neuron.2011.04.028 . .

J. Zhang , , , J.B. Ackman , , , H.P. Xu , , , 等 . . Visual map development depends on the temporal pattern of binocular activity in mice . . Nat. Neurosci. , , 2012 . . 15 ( ( 2 ): ): 298 - - 307 . . DOI: 10.1038/nn.3007 http://doi.org/10.1038/nn.3007 . .

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